《植物生理学报》 2012, 48(12): 1205-1209

枇杷叶荚蒾的愈伤组织诱导及植株再生

袁云香^1,2,*

1渭南师范学院化学与生命科学学院, 陕西渭南714099; 2陕西省多河流湿地生态环境重点实验室, 陕西渭南714099

通信作者：袁云香；E-mail: yuanyunxiang2006@126.com

摘　要：

以枇杷叶荚蒾幼叶为外植体, MS为基本培养基, 研究不同种类和浓度的植物生长调节物质对愈伤组织诱导、分化及 生根的影响, 建立了枇杷叶荚蒾再生体系。结果表明: 枇杷叶荚蒾最佳灭菌组合为75%酒精预处理20 s, 再用0.1% HgC12浸 泡12 min; 最佳愈伤组织诱导培养基为MS+6-BA 1.0 mg•L-1+NAA 0.25 mg•L-1+2,4-D 1.0 mg•L-1, 诱导率最高达92%; 最适芽 分化培养基为MS+6-BA 1.5 mg•L-1+NAA 0.2 mg•L-1, 分化率达87.25%; 适宜的生根培养基为1/2MS+NAA 1.0 mg•L-1, 生根率 达85%。

关键词：枇杷叶荚蒾; 愈伤组织诱导; 植株再生

收稿：2012-10-22   修定：2012-11-28

资助：国家自然科学基金项目(31000410)和陕西省教育厅项目(12JK0832和09JK434)。

Callus Induction and Plantlet Regeneration of Viburnum rhytidophyllum Hemsl.

YUAN Yun-Xiang^1,2,*

¹School of Chemistry and Life Science, Weinan Normal University, Weinan, Shaanxi 714099, China; ²Key Laboratory for EcoEnvironment of Multi-River Wetlands in Shaanxi Province, Weinan, Shaanxi 714099, China

Corresponding author: YUAN Yun-Xiang; E-mail: yuanyunxiang2006@126.com

Abstract:

The young leaves of Viburnum rhytidophyllum were taken as explants, and MS was basic medium. The effects of different concentrations and combinations of plant growth regulators on callus induction, bud differentiation and root formation were studied. Tissue culture regeneration system for V. rhytidophyllum was established. The results showed that the best sterilization method for materials was to soak explants into 0.1% HgC1₂ solution for 12 min after pretreatment with 75% alcohol for 20 s; the best medium for callus formation was MS+6-BA 1.0 mg·L^-1+NAA 0.25 mg·L^-1+2,4-D 1.0 mg·L^-1, callus induction rate reached 92%; the optimum bud differentiation medium was MS+6-BA 1.5 mg·L^-1+NAA 0.2 mg·L^-1with the induction rate of 87.25%; the suitable medium for root induction was 1/2MS+NAA 1.0 mg·L^-1 and the rooting rate was 85%.

Key words: Viburnum rhytidophyllum; callus induction; plant regeneration